Vspl methylase belongs to m6A-gamma class of adenine methylases

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Vspl methylase belongs to m6A-gamma class of adenine methylases.

Bacterial DNA-methyltransferases are responsible for site-specific modification of DNA. They transfer the methyl-group from Sadenosylmethionine (SAM) to DNA with formation of N6-methyladenosine (m6A), 5-methylcytosine (m5C) or N4-methylcytosine (m4Q (1). Lately nucleotide structures of more than one hundred genes of different methyltransferases have been determined (2). Analysis of deduced amin...

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SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases.

SpoU is encoded by the fourth open reading frame (ORF) of the spo operon of Escherichia coli (1). The 5'-terminal gene of this operon, gmk, encodes guanylate kinase; the second gene, rpoZ, encodes the w subunit of RNA polymerase; the third gene, spoT, encodes ppGpp hydrolase, and the 3'-terminal gene, recG, encodes a helicase-like protein involved in recombination (1 and references therein). Sp...

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Salmonella DNA adenine methylase mutants confer cross-protective immunity.

Salmonella isolates that lack or overproduce DNA adenine methylase (Dam) elicited a cross-protective immune response to different Salmonella serovars. The protection afforded by the Salmonella enterica serovar Typhimurium Dam vaccine was greater than that elicited in mice that survived a virulent infection. S. enterica serovar Typhimurium Dam mutant strains exhibited enhanced sensitivity to med...

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SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases.

Nucleotide modification is a major source of structural and biochemical diversity of RNAs, particularly of rRNAs and tRNAs (Rozenski et al., 1999). Basespecific methylation is one of the prevalent modes of post-transcriptional modification along with pseudouridylation and thio-uridylation. The catalytic domains of the majority of RNA methylases belong to the vast la rge superc lass o f Rossmanf...

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Salmonella DNA adenine methylase mutants elicit protective immune responses to homologous and heterologous serovars in chickens.

Salmonella DNA adenine methylase (Dam) mutants that lack or overproduce Dam are highly attenuated for virulence in mice and confer protection against murine typhoid fever. To determine whether vaccines based on Dam are efficacious in poultry, a Salmonella Dam(-) vaccine was evaluated in the protection of chicken broilers against oral challenge with homologous and heterologous Salmonella serovar...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1993

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/21.8.2015